The regulation of manganese superoxide dismutase and other antioxidant enzymes in Drosophila melanogaster

dc.contributor.advisorPhillips, J.P.
dc.contributor.advisorHilliker, A.J.
dc.contributor.authorLepp, Dion of Molecular and Cellular Biologyen_US of Guelphen_US of Scienceen_US
dc.description.abstractModulation of gene expression appears to be a common theme in the response to oxidative stress from bacteria to mammals. In this study, the transcriptional regulation of the 'Drosophila' genes Mn superoxide dismutase (MnSOD), Cu/ZnSOD and XDH was investigated. The expression of a MnSOD-' lacZ' fusion reporter gene was characterized in 'Drosophila ' adults and was found to differ from that of MnSOD transcript, indicating the possibilty of additional MnSOD regulatory elements outside the promoter region. No evidence for the involvement of the MAP kinase of Toll signaling pathways in the control of MnSOD basal transcription was discovered. Furthermore, there was no change in MnSOD transcript levels following treatment with bacterial LPS of with oxidative stress. Cu/ZnSOD and XDH were also unresponsive to oxidative stress, with the exception of a 2-fold induction of XDH in response to paraquat. Thus, there is no evidence that a co-ordinate response to oxidative stress occurs in 'Drosophila'.en_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectgene expressionen_US
dc.subjectoxidative stressen_US
dc.subjecttranscriptional regulationen_US
dc.subjectmanganese superoxide dismutaseen_US
dc.subjectantioxidant enzymesen_US
dc.subjectDrosophila melanogasteren_US
dc.titleThe regulation of manganese superoxide dismutase and other antioxidant enzymes in Drosophila melanogasteren_US


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