An optimized method for lab and industrial scale extraction of avocatin B

dc.contributor.advisorSpagnuolo, Paul
dc.contributor.authorAlghaysh, Manal
dc.date.accessioned2023-01-09T16:43:26Z
dc.date.available2023-01-09T16:43:26Z
dc.date.copyright2023-01
dc.date.created2022-12-15
dc.degree.departmentDepartment of Food Scienceen_US
dc.degree.grantorUniversity of Guelphen
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeFood Scienceen_US
dc.description.abstractAvocatin B (AVO) is an avocado derived compound with demonstrated bioactivity against many diseases. It exists in different compartments of the avocado fruit; including the seed, which is typically discarded. To valorize this waste stream, a sustainable method to extract AVO in large quantities was developed. The AVO-rich extracts were prepared by solvent-based extraction followed by saponification. The amount of AVO in enriched extracts were measured by LC/MS after each modification of extraction and saponification parameters. The developed method obtained promising results in both small and large scale, which helps to increase the yields for industrial applications. Cytotoxicity of these AVO-rich extracts in acute myeloid leukemia cells was confirmed. To complete the method, the AVO-rich extract was purified by consuming the minimum amounts of reagents needed for purification. This developed method represents a simple, accurate and feasible method to extract AVO from avocado seed.en_US
dc.identifier.urihttps://hdl.handle.net/10214/27390
dc.language.isoenen_US
dc.publisherUniversity of Guelphen
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectAvocatin Ben_US
dc.subjectAcetogeninsen_US
dc.subjectPolyhydroxylated fatty alcoholsen_US
dc.subjectPFAen_US
dc.subjectExtractionen_US
dc.subjectBioactive compoundsen_US
dc.titleAn optimized method for lab and industrial scale extraction of avocatin Ben_US
dc.typeThesisen
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