Surface Analytical Studies of Gramicidin A in Biomimetic Model Membrane Systems

dc.contributor.advisorLipkowski, Jacek
dc.contributor.authorGoodall, Barbara Lynn
dc.date.accessioned2014-12-24T18:17:29Z
dc.date.available2014-12-24T18:17:29Z
dc.date.copyright2014-12
dc.date.created2014-12-12
dc.date.issued2014-12-24
dc.degree.departmentDepartment of Chemistryen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeChemistryen_US
dc.description.abstractThis thesis is an investigation of the properties of gramicidin A in model membrane systems. This study investigates a solid supported bilayer lipid membrane comprised of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and gramicidin A deposited on a thioglucose modified Au(111) electrode. A “floating” bilayer lipid membrane was also investigated that was composed of DMPC, cholesterol, glycolipid monosialoganglioside (GM1), and gramicidin A deposited on a thioglucose modified Au(111) electrode. The GM1 was used as a spacer molecule to create a water reservoir between the modified electrode and lipid bilayer. Polarization modulation infrared reflection adsorption spectroscopy (PM-IRRAS) was used to investigate the solid supported and the floating bilayer lipid membrane. The PM-IRRAS studies were completed to probe the electric field driven changes in the orientation and conformation of gramicidin A in the lipid bilayer. Atomic force microscopy was used to investigate the topography and thickness of the floating bilayer lipid membrane system.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.identifier.urihttp://hdl.handle.net/10214/8635
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectgramicidinen_US
dc.subjectPM-IRRASen_US
dc.subjectAFMen_US
dc.subjectmodel membraneen_US
dc.titleSurface Analytical Studies of Gramicidin A in Biomimetic Model Membrane Systemsen_US
dc.typeThesisen_US

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