The Effect of Low Anatomical Temperatures on Oncolytic Rhabdoviruses
Cancers and infectious diseases are two of the most significant health problems globally and new therapeutics and prevention methods are being researched every day. Viral vectors are powerful tools that can be used to treat cancers and in the prevention of infectious diseases, in the form of oncolytic viruses (OVs) and viral vector-based vaccines, respectively. Rhabdoviruses have shown efficacy in in vitro and in pre-clinical studies as viral vectors. However, research investigating how environmental conditions affect their performance when used as therapeutics is limited. Preliminary data from our research group demonstrated that decreased temperatures could negatively affect the oncolytic ability of two rhabdoviruses, vesicular stomatitis virus (VSV) and Maraba virus. This thesis aimed to further investigate the relationship between lower temperatures and viral vector efficacy and how cold adaptation could be used as a mitigation strategy. The interface between virus biology, cell biology, and temperature is also discussed and investigated.