Regulation of Dynactin by Palmitoylation

dc.contributor.advisorSanders, Shaun
dc.contributor.authorLeekha, Arshia
dc.date.accessioned2022-12-08T13:58:03Z
dc.date.available2022-12-08T13:58:03Z
dc.date.copyright2022-12
dc.date.created2022-11-15
dc.degree.departmentDepartment of Molecular and Cellular Biologyen_US
dc.degree.grantorUniversity of Guelphen
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeMolecular and Cellular Biologyen_US
dc.description.abstractNeurons are large, complex cells that use a microtubule motor protein-based system for the fast transport of proteins and organelle cargo throughout long, complex projections. In output projections known as axons, cytoplasmic dynein transports cargo in the retrograde direction towards the cell body. Dynein requires the activating complex dynactin, including the subunit DCTN1 (p150glued). DCTN1 is post-translationally modified with the lipid palmitate in a process known as palmitoylation at cysteines 617 and 1255, likely by ZDHHC12. Additionally, DCTN1 is preferentially palmitoylated in the nervous system. In neurons, when wild type (wt) or palmitoylation resistant (C617,1255A; CCAA) are ectopically expressed, there may be more wtDCTN1-GFP in distal axons than DCTN1-CCAA-GFP. The results of this study provide a greater understanding of the regulation of transport in neurons with implications for synaptic function and neurodegeneration.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (RGPIN-2021-02547)
dc.identifier.urihttps://hdl.handle.net/10214/27317
dc.language.isoenen_US
dc.publisherUniversity of Guelphen
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectPalmitoylationen_US
dc.subjectNeuronsen_US
dc.subjectDynactinen_US
dc.subjectDyneinen_US
dc.titleRegulation of Dynactin by Palmitoylationen_US
dc.typeThesisen

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