Regulation of CYP17A1 activity and its potential implications on the development of boar taint

dc.contributor.advisorSquires, E.J.
dc.contributor.authorBillen, Machteld Joanna
dc.date.accessioned2020-08-24T15:45:59Z
dc.date.available2020-08-24T15:45:59Z
dc.date.copyright2008
dc.degree.departmentDepartment of Animal and Poultry Scienceen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractTesticular 16-androstene steroids, in particular 5[alpha]-androstenone, contribute to an off-odour and off-flavour known as boar taint. Cytochrome P450 C17 (CYP17A1) catalyses the key regulatory step in the formation of the 16-androstene steroids from pregnenolone via the andien-[beta] synthase reaction, or the synthesis of the glucocorticoid and sex steroids via 17[alpha]-hydroxylase and C17,20 lyase pathways respectively. The goals of this research were to investigate the effect of both isoforms of CYB5 and the phosphorylation status of CYP17A1 on these different activities of CYP17A1, by overexpressing these proteins in HEK-293 cells. CYB5A increased andien-[beta] synthase and C17,20 lyase activities while CYB5B increased C17,20 lyase but had no effect on andien-[beta] synthase activity. Phosphorylation of CYP17A1 at Ser 106 in the presence of CYB5A increased C17,20 lyase and andien-[beta] synthase activity. This suggests that decreased expression of CYB5A and increased expression of CYB5B would maintain sex steroid production and decrease boar taint. Key Words: CYP17A1, CYB5, Androstenone, C17, 20 lyase, 17[alpha]-hydroxylaseen_US
dc.identifier.urihttps://hdl.handle.net/10214/19998
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectBoar tainten_US
dc.subjectSteroidsen_US
dc.subject16-androsteneen_US
dc.subjectCYP17A1en_US
dc.subjectPregnenoloneen_US
dc.titleRegulation of CYP17A1 activity and its potential implications on the development of boar tainten_US
dc.typeThesisen_US

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