Evaluation of a Potential Synbiotic Against a Cronobacter sakazakii Cocktail in a Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) Using Infant Fecal Matter

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dc.contributor.advisorGoodridge, Lawrence
dc.contributor.authorKe, Alfred
dc.date.accessioned2022-04-14T20:20:18Z
dc.date.available2022-04-14T20:20:18Z
dc.date.copyright2022-04
dc.date.created2022-03-16
dc.degree.departmentDepartment of Food Scienceen_US
dc.degree.grantorUniversity of Guelphen
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeFood Scienceen_US
dc.description.abstractPowdered infant formula (PIF) can be contaminated with Cronobacter sakazakii, which can cause severe illnesses in infants. Synbiotics could act as an alternative control measure for C. sakazakii contamination in PIF and within the infant gut, but synbiotics have not been well studied for their ability to inhibit C. sakazakii. This thesis demonstrated that a potential synbiotic, consisting of 6 LAB strains and Vivinal GOS, can inhibit the growth of C. sakazakii in an infant SHIME, possibly through the production of antimicrobial metabolites like acetate, increasing species diversity within the system to compete for nutrients, or a combination of mechanisms. Our results also revealed that the relative abundance of Bifidobacterium was negatively correlated with Cronobacter in the control SHIME (ρ = -0.96; p < 0.05). This study suggests that C. sakazakii could be inhibited by the native gut microbiota, but inhibition can be accelerated by a potential synbiotic treatment.en_US
dc.description.sponsorshipCanada First Research Excellence Fund
dc.identifier.citationKe, A., Parreira, V. R., Goodridge, L., and Farber, J. M. (2021). Current and Future Perspectives on the Role of Probiotics, Prebiotics, and Synbiotics in Controlling Pathogenic Cronobacter Spp. in Infants. Frontiers in Microbiology, 12, 3158. https://doi.org/10.3389/fmicb.2021.755083
dc.identifier.urihttps://hdl.handle.net/10214/26858
dc.language.isoenen_US
dc.publisherUniversity of Guelphen
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectSynbioticen_US
dc.subjectCronobacter sakazakiien_US
dc.subjectSimulator of the Human Intestinal Microbial Ecosystem (SHIME)en_US
dc.subjectMetabolomicsen_US
dc.subjectGut microbiotaen_US
dc.subjectInfanten_US
dc.titleEvaluation of a Potential Synbiotic Against a Cronobacter sakazakii Cocktail in a Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) Using Infant Fecal Matteren_US
dc.typeThesisen
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