There is a lack of research regarding dietary supplement use among Gay, Bisexual, Transgender, Two-spirit, Queer (GBT2Q) men and non-binary individuals. This research aims to investigate patterns and predictors of dietary supplement use among this population. An online questionnaire was completed by 204 participants (52.5% men, 43.1% gay, mean age 29.34  6.77) who were consuming supplements at the time of recruitment. Overall, the most consumed supplements were vitamins/minerals (92.2%), proteins (84.3%), and carbohydrates (77.5%). Our findings revealed that identifying as certain genders and sexualities was significantly associated with the types of dietary supplements consumed. Most common reason for consuming supplements was to improve immunity and most common source of information was health professionals. This investigation indicates the importance of educating health care professionals regarding the safe use of dietary supplements and concludes the importance of identifying predictors of dietary supplement use to inform adequate policymaking and research.