Proglycogen and macroglycogen in equine skeletal muscle
The objectives of this thesis were to investigate the existence of proglycogen (PG) and macroglycogen (MG) in equine skeletal muscle; to compare the analysis of total glycogen by two methods (acid hydrolysis and MG + PG determination) for total muscle glycogen from biopsy sized samples and to evaluate the effect of extraction time and perchloric acid (PCA) concentration on the recovery of MG and PG. The first study indicated that extraction time had very little effect on the recovery of PG and MG but there was an effect of PCA concentration. Extraction with 0.5 and 1.5 M PCA yielded similar values for PG and MG but extraction in 3.0 M PCA yielded lower PG and higher MG concentrations (P < 0.05). The results from the second study showed that PG and MG exist in equine skeletal muscle and the measurement techniques for total glycogen analyzed by the two methods were very comparable.