Amide proton assignments of partially deuterated Anabaena Sensory Rhodopsin, investigation of solvent exchangeability and deuterium isotope effects




Bolton, David

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University of Guelph


Magic angle spinning solid-state NMR has been used with great success in studying membrane proteins in a native-like environment. These typically carbon-detected experiments are very time consuming, require a large amount of multiple samples with various levels of isotopic labeling. Through the use of proton detected experiments coupled with fast MAS we can reduce the total experimental time and number of required samples. Technological developments and labelling techniques (ultrafast MAS and isotopic dilution with 2H) have made proton detection in solid-state NMR a powerful tool. Membrane proteins however, remain a difficult subject of study. In this thesis, assignments of both exchangeable and non-exchangeable amide protons were made. Solvent accessibility as well as deuterium isotope effects on chemical shifts were evaluated to help the assignment and adjustment of assignment of the chemical shifts of proteins in future studies.



solvent accessibility, deuterium isotope, Anabaena sensory rhodopsin, amide protons