Evaluation of a simulated pneumoperitoneum environment on cancer cell growth using an in vitro model with positive pressure carbon dioxide insufflation and canine transitional cell carcinoma
Laparoscopy is routinely performed in canine patients with cancer and the effects of CO2 insufflation on growth and dissemination of neoplastic cells are unknown. Canine TCC and MDCK cells were exposed to 100% CO2 at various pressures for 2 hours using an insufflator at 37°C in vitro. Culture media pH was measured. Viability, proliferation and migration were assessed using Resazurin, Trypan Blue Dye, and Scratch Migration assays, respectively up to 7 days post CO2. ANOVA were used for statistical analyses. pH significantly decreased immediately after CO2 exposure. Various CO2 exposure pressures significantly influenced viability with a nonlinear trend in effect. CO2 pressure had no significant effects on proliferation and migration. In conclusion, a positive pressure CO2 environment causes a significant change in cell viability of canine TCC and MDCK cells without affecting their proliferation or migration up to 7 days post CO2. Longer term effects are unknown.