Detecting Subarctic Caddisflies using eDNA and Measuring the Phylogenetic Signal of their Choice in Habitat

dc.contributor.advisorAdamowicz, Sarah
dc.contributor.authorChatila-Amos, Kamil
dc.date.accessioned2021-05-12T15:25:13Z
dc.date.available2021-05-12T15:25:13Z
dc.date.copyright2021-04
dc.date.created2021-04-13
dc.degree.departmentDepartment of Integrative Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeIntegrative Biologyen_US
dc.description.abstractEnvironmental DNA (eDNA) analysis is a powerful new tool for biomonitoring applications, with potential applications in entomology. This study evaluated the utility of a newly developed qPCR assay designed to detect eDNA from a species of an aquatic benthic macroinvertebrate (Philarctus bergrothi) in Churchill, Manitoba. To inform the design of our eDNA-based assays, we investigated if habitat choice is conserved phylogenetically by sampling 53 species of caddisflies (Trichoptera). We found a high propensity for habitat switching and no significant phylogenetic signal for habitat choice within the caddisfly larvae of Churchill. When applying our novel molecular methods, we successfully detected P. bergrothi eDNA in 96% of the sites where evidence of the organism was found using conventional sampling. Upon evaluating the impacts of environmental characteristics on detection, no significant relationship was observed between environmental characteristics and the amount of target eDNA detected.en_US
dc.description.sponsorshipNorthern Scientific Training Program
dc.description.sponsorshipChurchill Northern Studies Centre (CNSC)
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipIdea to Innovation
dc.description.sponsorshipThe Arrell Food Institute
dc.description.sponsorshipW. Garfield Weston Foundation
dc.description.sponsorshipUniversity of Guelph
dc.identifier.urihttps://hdl.handle.net/10214/25718
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjecteDNAen_US
dc.subjectTrichopteraen_US
dc.subjectSubarcticen_US
dc.subjectPhylogenyen_US
dc.subjectEnvironmental DNAen_US
dc.subjectCaddisfliesen_US
dc.subjectChurchill, MBen_US
dc.titleDetecting Subarctic Caddisflies using eDNA and Measuring the Phylogenetic Signal of their Choice in Habitaten_US
dc.typeThesisen_US

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