Development of low-saturate Brassica napus L. doubled haploids

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Beaith, Michelle Erin

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University of Guelph

Abstract

High levels of saturated fatty acids (<7%) in Canadian canola oil over the past decade has created a demand for cultivars with reduced saturates in the seed oil. Ultraviolet treatment of microspores was used to induce mutations to reduce cotyledonary saturate levels in microspore-derived embryos. Maturation of embryos at an elevated temperature favoured the accumulation of saturated fatty acids and the identification of 'low saturate mutants'. Saturate levels (16:0+18:0+20:0+22:0+24:0) in mutant embryos ranged from 4.8 to 32.6% compared to approximately 11% in non-mutated embryos. Seed analysis of doubled haploids and increased doubled haploids revealed that 70 and 61%, respectively, exhibited reduced seed saturate phenotypes from the parent material, and 12 and 14%, respectively, produced seed with less than 7% saturates. The ten most stable mutant lines exhibited saturate variation of only 0.1 to 1.1 percentage points over three or four generations of controlled environment and field evaluation.

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Canadian anola oil, Reduced saturates, Low saturate mutants, Doubled haploids, Fatty acids

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