Development of low-saturate Brassica napus L. doubled haploids

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Beaith, Michelle Erin

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University of Guelph


High levels of saturated fatty acids (<7%) in Canadian canola oil over the past decade has created a demand for cultivars with reduced saturates in the seed oil. Ultraviolet treatment of microspores was used to induce mutations to reduce cotyledonary saturate levels in microspore-derived embryos. Maturation of embryos at an elevated temperature favoured the accumulation of saturated fatty acids and the identification of 'low saturate mutants'. Saturate levels (16:0+18:0+20:0+22:0+24:0) in mutant embryos ranged from 4.8 to 32.6% compared to approximately 11% in non-mutated embryos. Seed analysis of doubled haploids and increased doubled haploids revealed that 70 and 61%, respectively, exhibited reduced seed saturate phenotypes from the parent material, and 12 and 14%, respectively, produced seed with less than 7% saturates. The ten most stable mutant lines exhibited saturate variation of only 0.1 to 1.1 percentage points over three or four generations of controlled environment and field evaluation.



Canadian anola oil, Reduced saturates, Low saturate mutants, Doubled haploids, Fatty acids