Investigation of the Expression of the O:54 antigen from Salmonella enterica

dc.contributor.advisorWhitfield, Chris
dc.contributor.authorHunt, Brittany
dc.date.accessioned2013-11-18T18:55:43Z
dc.date.available2013-11-18T18:55:43Z
dc.date.copyright2013
dc.date.created2013-11-05
dc.date.issued2013-11-18
dc.degree.departmentDepartment of Molecular and Cellular Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeMolecular and Cellular Biologyen_US
dc.description.abstractWbbF is a proposed member of the synthase family of dual glycosyltransferase-exporter proteins. WbbF assembles the O:54 antigen from Salmonella enterica. The wb*O:54 operon (encoding wbbE, wbbF and mnaA) is found on a naturally-occuring plasmid (pWQ799). Previous research suggested only WbbE and WbbF are necessary for O:54 antigen assembly due to the presence of a chromosomal gene encoding WecB, a functional homolog of MnaA. WecB and MnaA synthesize the essential UDP-ManNAc donor. However, this study determined optimal production of O:54 in E. coli was dependent on the presence of MnaA. A growth defect was observed in cells producing O:54 antigen, but unable to ligate it to the lipopolysaccharide acceptor (due to ΔwaaL). Cells recovered growth over time via mutations that turned off (or down) O:54 antigen biosynthesis. During this study, a cysteine-free version of WbbF that retained function was established, providing a key resource for future topology mapping experiments.en_US
dc.identifier.urihttp://hdl.handle.net/10214/7624
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectO-antigenen_US
dc.subjectlipopolysaccharideen_US
dc.subjectsynthaseen_US
dc.subjectO:54en_US
dc.subjectWbbFen_US
dc.titleInvestigation of the Expression of the O:54 antigen from Salmonella entericaen_US
dc.typeThesisen_US

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