Investigating a role for phospholipids in plastid pleomorphy

dc.contributor.advisorMathur, Jaideep
dc.contributor.authorAnderson, Cole
dc.date.accessioned2018-05-09T20:53:48Z
dc.date.available2019-04-19T05:00:26Z
dc.date.copyright2018-04
dc.date.created2018-04-19
dc.date.issued2018-05-09
dc.degree.departmentDepartment of Molecular and Cellular Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeMolecular and Cellular Biologyen_US
dc.description.abstractPlastids, a defining feature of plants, produce stroma-filled extensions or tubules known as stromules. Although stromules are reliably induced upon exogenous sucrose treatment and inhibited upon silver nitrate treatment, a clear mechanism and function behind this phenomenon remains to be elucidated. The lack of inorganic phosphate appears to affect stromule levels as well as simultaneously cause a conversion of extraplastidic membrane lipids from phospholipids to galactolipids, suggesting a lipid-based mechanism for their formation. Non-specific phospholipase C 4 and non-specific phospholipase C 5, while responsible for this conversion, do not affect stromule formation. The origin of plastidial phosphatidylcholine upstream of phospholipid to galactolipid conversion is likely due to the presence of plastid associated membranes and does not play a role in stromule formation. The observations from my microscopy based studies demonstrate the organelle pleomorphy influencing ability of lysophosphatidylcholine, implicating acyl-editing, better known as the Lands cycle, in stromule formation.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.identifier.urihttp://hdl.handle.net/10214/13039
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectplastiden_US
dc.subjectchloroplasten_US
dc.subjectPLA2en_US
dc.subjectphospholipase A2en_US
dc.subjectPCen_US
dc.subjectphosphatidylcholineen_US
dc.subjectLPCen_US
dc.subjectlysophosphatidylcholineen_US
dc.subjectLPCATen_US
dc.subjectlysophosphatidylcholine acyltransferaseen_US
dc.titleInvestigating a role for phospholipids in plastid pleomorphyen_US
dc.typeThesisen_US

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