Causal agent, biology and management of the leaf and stem disease of boxwood (Buxus spp.)

dc.contributor.advisorHsiang, T.
dc.contributor.authorShi, Fang of Environmental Scienceen_US of Guelphen_US of Scienceen_US
dc.description.abstractAn outbreak of a boxwood disease was observed in Southern Ontario nurseries in 2008, but appears to have been present in Ontario for at least 10 years. From 2008 to 2010, over fungal 312 isolates were obtained from diseased samples. Eight major fungal morphotypes associated with the blight were identified with morphological and molecular techniques. Among them, only 'Volutella buxi' successfully satisfied Koch's postulates, which confirmed it is the causal agent of boxwood blight. Wounds may be the major penetration points for 'V. buxi' since non-wounded inoculated tissues did not become diseased. The 'Buxus' cultivar 'Green Gem' was the most susceptible compared to 'Green Velvet', 'Green Mound', or 'Green Mountain'. ISSR markers revealed low genetic variation of V buxi between different cultivars and locations indicating asexual reproduction and probably a recent origin in Canada. Six fungicidal treatments showed strong preventive activity and some curative activity against Volutella blight.en_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectleaf and stem diseaseen_US
dc.subjectcausal agenten_US
dc.subjectboxwood blighten_US
dc.subjectVolutella blighten_US
dc.titleCausal agent, biology and management of the leaf and stem disease of boxwood (Buxus spp.)en_US


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