Using UV light for the inactivation of pathogens in food and beverages has shown good results qualitatively, economically, and sustainably. In this research, UV light was used to obtain the D10 values of 13 strains of Cronobacter spp. in buffer using a collimated beam device. The D10 values ranged from 1.88 to 3.01 mJ/cm2. These D10 values were determined using biodosimetry studies and establishes Cronobacter spp. D10 range for future UV studies. In the second part of this study, a five-strain cocktail of C. sakazakii suspended in 2% partly skimmed milk was treated with UV light at different flow rates obtained by using a Laminar flow-through reactor. A total reduction of 2.47 +/- 0.10 logs was achieved at a flow rate of 10 mL/min. This study provides useful information that could be helpful in establishing the efficacy of UV light for inactivating C. sakazakii in commercial milk.