In vitro stressor related changes in ovarian steroidogenesis in rainbow trout (Oncorhynchus mykiss)

dc.contributor.advisorLeatherland, J.F.
dc.contributor.advisorKing, W.A.
dc.contributor.authorBarkataki, Sangjucta
dc.date.accessioned2020-08-24T15:44:56Z
dc.date.available2020-08-24T15:44:56Z
dc.date.copyright2007
dc.degree.departmentDepartment of Biomedical Sciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractThe study examined the affect of cortisol on basal and cAMP-stimulated ' in vitro' steroidogenesis of rainbow trout ('Oncorhynchus mykiss ') ovarian follicles at different maturational stages. Basal and cAMP-stimulated 17[beta]-estradiol (E2) and testosterone (T) production was highest during the mid-vitellogenic (MV) stage, compared with early- and late-vitellogenic stages. Cortisol suppressed the basal steroid production in the MV stage, and suppressed cAMP-stimulated steroid production in all three maturational stages. To determine whether cortisol exerts its effect on pregnenolone (P 5) production, real time RT-PCR was used to measure the expression of genes coding for steroidogenic acute regulatory (StAR) protein, and cytochrome P450scc. There was no significant affect of cortisol on the expression of either gene. To look for possible actions of cortisol on steroidogenic enzyme function, the effect of the glucocorticoid on the biotransformation of radiolabelled P5, and T was examined; cortisol appeared to suppress the aromatization of androgens to estrogens by a mechanism that is not fully understood.en_US
dc.identifier.urihttps://hdl.handle.net/10214/19879
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectRainbow trouten_US
dc.subjectCortisolen_US
dc.subjectSteroidogenesisen_US
dc.subjectOvarianen_US
dc.subjectTestosteroneen_US
dc.titleIn vitro stressor related changes in ovarian steroidogenesis in rainbow trout (Oncorhynchus mykiss)en_US
dc.typeThesisen_US

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