The Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus)

dc.contributor.advisorParker, Linda
dc.contributor.authorO'Brien, Lesley D
dc.date.accessioned2013-08-07T13:52:37Z
dc.date.available2013-08-07T13:52:37Z
dc.date.copyright2013-07
dc.date.created2013-07-17
dc.date.issued2013-08-07
dc.degree.departmentDepartment of Psychologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmePsychologyen_US
dc.description.abstractConsiderable evidence supports anandamide (AEA) as an important mediator in the regulation of nausea and vomiting. The present study investigates the effect of inhibiting a protein reported to mediate AEA transport, FLAT (FAAH-1-like AEA transporter), on nausea and vomiting and the neural correlates of AEA regulated nausea in the visceral insular cortex (VIC). The systemic administration of the AEA transport inhibitor ARN272 was evaluated in LiCl-induced conditioned gaping in rats, and vomiting in shrews. The effect of intra-cranial administration of ARN272 into the VIC was also investigated using LiCl-induced conditioned gaping in rats. Systemic administration of ARN272 dose-dependently suppressed LiCl-induced conditioned gaping in rats, and was reversed by CB1 receptor antagonism with SR141716. Systemic administration of ARN272 also attenuated vomiting in shrews. Delivery of ARN272 into the VIC produced no effect on LiCl-induced conditioned gaping in rats. These results suggest that preventing the cellular reuptake of AEA through transport inhibition tonically activates CB1 receptors to regulate toxin-induced nausea, but that this is not AEA regulated within the VIC.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.identifier.urihttp://hdl.handle.net/10214/7296
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectEndocannabinoiden_US
dc.subjectAnandamideen_US
dc.subjectNauseaen_US
dc.subjectGapingen_US
dc.subjectVomitingen_US
dc.subjectFAAH-1-like anandamide transporteren_US
dc.subjectFLATen_US
dc.subjectCB1en_US
dc.subjectARN272en_US
dc.subjecttaste reactivityen_US
dc.subjectVisceral Insular Cortexen_US
dc.titleThe Impact of Prolonged Anandamide Availability by Anandamide Transport Inhibition on Nausea-Induced Behaviour in Rats and Vomiting in Shrews (Suncus murinus)en_US
dc.typeThesisen_US

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