Identification and phylogenetic relationships within the genus Tetrahymena using the cytochrome c oxidase subunit 1 gene and the small subunit ribosomal RNA gene

dc.contributor.advisorLynn, Denis H.
dc.contributor.authorChantangsi, Chitchai
dc.date.accessioned2020-08-24T15:49:35Z
dc.date.available2020-08-24T15:49:35Z
dc.date.copyright2006
dc.degree.departmentDepartment of Zoologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractNucleotide sequences of the mitochondrial cytochrome 'c' oxidase subunit 1 ('cox1') gene were used as a "DNA barcode" to identify the closely related species of the ciliate genus 'Tetrahymena ' and 3 other tetrahymenine species. Except for 'Tetrahymena furgasoni, Tetrahymena lwoffi, Tetrahymena pyriformis', and ' Tetrahymena setosa', all isolates could be identified to the species level using 689 bp of the 'cox1' sequences. Intraspecific isolates of 'Tetrahymena borealis, T. lwoffi, Tetrahymena patula', and ' Tetrahymena thermophila' could be discriminated and identified by their 'cox1' sequences as shown by <0.65% intraspecific sequence divegences. However, conspecific individuals of 'T. pyriformis ' and 'Tetrahymena tropicalis' showed high "within-species" sequence divergence values of 5.01% and 9.07%, respectively. Phylogenetic trees constructed using SSUrRNA and 'cox1' nucleotide and amino acid sequences showed the genus 'Tetrahymena' to be monophyletic as opposed to three morphology-and-ecology-based groupings, which are paraphyletic. The SSUrRNA phylogeny showed the 9 previously established ribosets. The 'cox1' nucleotide trees revealed 12 well supported groupings, called coxisets, which mostly corresponded to the 9 ribosets.en_US
dc.identifier.urihttps://hdl.handle.net/10214/20277
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectDNA barcodingen_US
dc.subjectciliateen_US
dc.subjectTetrahymenaen_US
dc.subjecttetrahymenine speciesen_US
dc.subjectcytochrome c oxidase subunit 1 geneen_US
dc.titleIdentification and phylogenetic relationships within the genus Tetrahymena using the cytochrome c oxidase subunit 1 gene and the small subunit ribosomal RNA geneen_US
dc.typeThesisen_US

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