Dissecting Dlx5- and Dlx6-mediated regulation of limb chondrogenesis: characterization of functional domains

dc.contributor.advisorBendall, A.
dc.contributor.authorHsu, Shu-hsuan Claire
dc.date.accessioned2020-12-03T18:09:35Z
dc.date.available2020-12-03T18:09:35Z
dc.date.copyright2004
dc.degree.departmentDepartment of Molecular Biology and Geneticsen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractIn the early limb bud, 'Dlx5' and 'Dlx6', members of the 'Dlx' homeobox gene family, are expressed in condensed mesenchymal cells that differentiate into chondrocytes. Analyses of 'Dlx5' and compound 'Dlx5'/'6' mutant mice reveal that 'Dlx5' and 'Dlx6' positively regulate chondrocyte differentiation in a functionally redundant manner. We misexpressed 'Dlx5' and 'Dlx6' in chicken embryo limb bud mesenchymal cells both 'in vitro' and 'in vivo ' to map the functional domains of their chondrogenic stimulatory function. The amino-terminal domain of Dlx5 was required for its chondrogenic stimulatory function. In contrast to Dlx5, both the amino- and carboxy-terminal domains of Dlx6 were required for this conserved function. Results of the functional domain mapping of Dlx5 'in vivo' were consistent with the in vitro data, and misexpression of 'Dlx5' or ' Dlx6 in vivo' resulted in reduced ossification of all skeletal elements in the wing.en_US
dc.identifier.urihttps://hdl.handle.net/10214/22033
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectearly limb buden_US
dc.subjectDlx5en_US
dc.subjectDlx6en_US
dc.subjectDlx homeobox gene familyen_US
dc.subjectmesenchymal cellsen_US
dc.subjectchondrocytesen_US
dc.subjectchondrocyte differentiationen_US
dc.subjectregulationen_US
dc.titleDissecting Dlx5- and Dlx6-mediated regulation of limb chondrogenesis: characterization of functional domainsen_US
dc.typeThesisen_US

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