The Role of Exercise-Mediated Mitochondrial ROS Production in the Regulation of Oxidative Phosphorylation

dc.contributor.advisorHolloway, Graham
dc.contributor.authorBarbeau, Pierre-Andre
dc.date.accessioned2017-09-12T19:06:16Z
dc.date.available2018-08-29T05:00:20Z
dc.date.copyright2017-08
dc.date.created2017-08-29
dc.date.issued2017-09-12
dc.degree.departmentDepartment of Human Health and Nutritional Sciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeHuman Health and Nutritional Sciencesen_US
dc.description.abstractThe transport of long chain fatty acids (LCFA) and ADP into the mitochondria are two major control points of oxidative phosphorylation that can be upregulated during exercise in order to maintain cellular ATP demand. The rate-limiting steps of LCFA and ADP transport are thought to be sensitive to redox modification, suggesting the importance of exercise-mediated mitochondrial ROS production. Therefore, we used MCAT mice, which have human catalase targeted to the mitochondria, to determine if decreasing mitochondrial ROS emission affects oxidative phosphorylation. Attenuating mitochondrial ROS significantly reduced exercise capacity in the absence of alterations in mitochondrial content. While an acute bout of exercise decreased ADP sensitivity by ~30% in WT mice, MCAT mice retained ADP sensitivity. In contrast, while exercise decreased MCoA-mediated inhibition of lipid-supported respiration, the responses within WT and MCAT mice were similar. Altogether, these findings demonstrate that exercise-mediated mitochondrial ROS emission regulates ADP sensitivity, but not lipid-supported respiration.en_US
dc.identifier.urihttp://hdl.handle.net/10214/11578
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rightsAttribution-NoDerivs 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nd/2.5/ca/*
dc.subjectMitochondriaen_US
dc.subjectMitochondrial ADP transporten_US
dc.subjectMitochondrial LCFA transporten_US
dc.subjectRedox modificationen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectMCAT mouseen_US
dc.subjectOxidative phosphorylationen_US
dc.subjectCellular respirationen_US
dc.titleThe Role of Exercise-Mediated Mitochondrial ROS Production in the Regulation of Oxidative Phosphorylationen_US
dc.typeThesisen_US

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