Regulation of human CTP: Ethanolaminephosphate cytidylyltransferase (PCYT2) by lipids and serum

dc.contributor.advisorBakovic, Marica
dc.contributor.authorZhu, Lin
dc.date.accessioned2021-03-31T15:37:34Z
dc.date.available2021-03-31T15:37:34Z
dc.date.copyright2004
dc.degree.departmentDepartment of Human Biology and Nutritional Sciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractPhosphatidylethanolamine (PE) is an essential membrane phospholipid and important supplier of biologically active lipid-derived molecules. The present study includes various aspects of regulation of the CDP-ethanolamine pathway and the rate-controlling enzyme, Pcyt2, in the 'de novo' biosynthesis of PE in human breast cancer cells, MCF-7. Both mouse and human Pcyt2 promoters and human PCYT2 mRNA levels are down-regulated by serum lipids and delipidated serum (serum growth factors), and in contrast significantly up-regulated in the absence of lipids and serum. Fatty acids, oleic acid and EPA added to the delipidated serum media significantly inhibit the PCYT2 promoter and enzyme activity, which is likely driven at the transcriptional level. 25-OH-cholesterol was a strong inhibitor of the PCYT2 promoter-luciferase activity in transient expression experiments. However, the promoter assay did not correspond to the changes in endogenous PCYT2 activity and it is possible that multiple mechanisms are involved in its regulation.en_US
dc.identifier.urihttps://hdl.handle.net/10214/24306
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectphosphatidylethanolamineen_US
dc.subjectregulationen_US
dc.subjectCDP-ethanolamine pathwayen_US
dc.subjectrate-controlling enzymeen_US
dc.subjectPcyt2en_US
dc.subjectbiosynthesisen_US
dc.subjecthuman breast cancer cellsen_US
dc.titleRegulation of human CTP: Ethanolaminephosphate cytidylyltransferase (PCYT2) by lipids and serumen_US
dc.typeThesisen_US

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