Biocontrol of foodborne bacterial pathogens using immobilized bacteriophages

dc.contributor.advisorGriffiths, M.W.
dc.contributor.authorAnany, Hany El-Said Mohamad
dc.date.accessioned2020-08-24T15:43:37Z
dc.date.available2020-08-24T15:43:37Z
dc.date.copyright2010
dc.degree.departmentDepartment of Food Scienceen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameDoctor of Philosophyen_US
dc.description.abstractThe goal of the present research was to develop a simple technique to immobilize isolated lytic phages and explore the potential use of these immobilized phages to control certain foodborne pathogens in a real food system. More than one hundred phages were isolated from different environmental samples against different strains of four major foodborne pathogens; 'E. coli ' O157:H7, 'Salmonella, Listeria monocytogenes' and ' Shigella'. A turbidimetric method in high throughput format using the Bioscreen C was used to monitor phage lytic activity and determine the host range of the isolated phages. This enabled identification of isolated phages with similar characteristics and the selection of twelve good phage candidates for biocontrol purposes. These phages were characterized by TEM, restriction endonuclease pattern, one-step growth curve, BIM (bacteriphage insensitve mutant) development, cross infectivity and determination of their stability and infectivity under different conditions. A novel 'Shigella ' phage, [Phi]SboM-AG3, was isolated and its genome sequenced. Its genome did not show any homology to any reported virulent or lysogenic genes and it was considered as a member of the "T4 superfamily". Phage cocktails made from the isolated phages were very effective for specific control of the four target pathogens in both broth media and contaminated food stored under different environmental conditions. The phages were immobilized using positively charged carrier substrates, which allowed specific 'binding of phages through their heads and leaving tail fibers free to interact with bacteria. These immobilized phages retained infectivity and immobilized 'Listeria ' and 'E. coli' phage cocktails were able to control the growth of 'L. monocytogenes' and 'E. coli' O157:H7, respectively, in food under different temperatures and packaging conditions.en_US
dc.identifier.urihttps://hdl.handle.net/10214/19666
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectBiocontrolen_US
dc.subjectSalmonellaen_US
dc.subjectListeriaen_US
dc.subjectShigellaen_US
dc.subjectEscherichia colien_US
dc.titleBiocontrol of foodborne bacterial pathogens using immobilized bacteriophagesen_US
dc.typeThesisen_US

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