Mapping black spot disease resistance and cold hardiness in garden roses (Rosa x hybrida)

dc.contributor.advisorLee, Elizabeth
dc.contributor.authorRouet, Cindy
dc.date.accessioned2021-05-12T15:16:25Z
dc.date.copyright2021-04
dc.date.created2021-04-26
dc.date.issued2019-09
dc.degree.departmentDepartment of Plant Agricultureen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameDoctor of Philosophyen_US
dc.degree.programmePlant Agricultureen_US
dc.description.abstractThis thesis aims to contribute to the modernization of Canada’s national rose breeding program by developing molecular markers associated with traits of major interest. Roses are one of the most economically important ornamental crops. Consumers’ preferences are continuously evolving, meaning that the rose industry needs to release new varieties at a fast pace to remain competitive. As a consensus, consumers want low maintenance roses. To meet consumers’ demand, Canada’s national rose breeding program, which is hosted in the Niagara Region, focuses on breeding for black spot disease resistance and winter hardiness. In this regard, Canadian Explorer roses are known worldwide for their exceptional hardiness. The objective of this thesis was to identify quantitative trait loci (QTL) associated with resistance to black spot disease (Diplocarpon rosae) and winter hardiness in polyploid bi-parental populations derived from Explorer roses, and to set up a framework for the development of molecular markers and the implementation of marker-assisted selection. Molecular tools not only have the potential to improve the accuracy and speed of the selection process, but they also promise to reduce the cost and the labor associated with recording complex phenotypic traits. This research identified a major QTL associated with the resistance to several races of Diplocarpon rosae under natural and artificial conditions on linkage group 1 of the rose genome for which a diagnostic marker was designed and validated in the genetic background of the parental donor. Furthermore, this research identified several QTLs associated with winter damage, spring regrowth and freezing tolerance measured by electrolyte leakage in artificial conditions. While no diagnostic markers were developed, this research highlighted the limited utility of electrolyte leakage as a proxy for field winter hardiness and the complexity of this phenotypic trait. Together, these results demonstrate the potential for implementing marker-assisted selection for black spot disease resistance in a rose breeding program, and provide a starting point for the molecular characterization of the genetic and molecular basis of winter hardiness in roses.en_US
dc.description.embargo2021-04-26
dc.description.sponsorshipVineland Research and Innovation Centre
dc.description.sponsorshipAgriculture and Agri-Food Canada Growing Forward 2 project #AIP-P013
dc.description.sponsorshipCanadian Agricultural partnership
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipOntario Ministry of Agriculture, Food and Rural Affairs
dc.description.sponsorshipCanadian Nursery Landscape Association
dc.description.sponsorshipLandscape Manitoba and Landscape Alberta
dc.description.sponsorshipInternational Tuition Scholarship from Ontario Agriculture College (OAC)
dc.description.sponsorshipOAC Internal Awards
dc.identifier.citationRouet, C., Lee, E.A., Banks, T. et al. Identification of a polymorphism within the Rosa multiflora muRdr1A gene linked to resistance to multiple races of Diplocarpon rosae W. in tetraploid garden roses (Rosa�????�??hybrida). Theor Appl Genet 133, 103�??117 (2020). https://doi.org/10.1007/s00122-019-03443-9
dc.identifier.urihttps://hdl.handle.net/10214/25716
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectRosesen_US
dc.subjectBlack spot diseaseen_US
dc.subjectWinter hardinessen_US
dc.subjectQTLen_US
dc.subjectRose breedingen_US
dc.subjectMarker assisted selectionen_US
dc.titleMapping black spot disease resistance and cold hardiness in garden roses (Rosa x hybrida)en_US
dc.typeThesisen_US

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