Mammary mTOR activity during intravenous glucose infusions into lactating dairy cows

dc.contributor.advisorCant, John
dc.contributor.authorCurtis, Richelle
dc.date.accessioned2013-09-09T15:48:05Z
dc.date.available2013-09-09T15:48:05Z
dc.date.copyright2013-07
dc.date.created2013-07-31
dc.date.issued2013-09-09
dc.degree.departmentDepartment of Animal and Poultry Scienceen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeAnimal and Poultry Scienceen_US
dc.description.abstractThe effects of glucose on milk protein yield and mammary mTOR activity were studied in eight multiparous early lactation Holstein cows fed a TMR with 40% NDF content. Treatments were jugular infusions of either physiological saline (SAL) or approximately 900 g/d glucose (GLC) in a cross over design for two 6 d periods during which blood, milk and mammary tissue were sampled. GLC infusion increased plasma insulin but did not significantly increase plasma glucose and only tended to increase milk protein yields. NEAA and BCAA plasma concentrations significantly increased and decreased, respectively, with GLC treatments. Furthermore, reduction of the phosphorylation state of mTOR signalling protein 4EBP1, as well as reduction of total and phosphorylated S6K1 abundances resulted from the infusion of GLC. The expected increase in milk protein synthesis due to glucose infusion was not observed, presumably because protein synthesis was stimulated in muscle tissue rather than the mammary glands.en_US
dc.description.sponsorshipDairy Farmers of Canada
dc.description.sponsorshipAgriculture and Agri-Food Canada
dc.description.sponsorshipOntario Ministry of Agriculture, Food and Rural Affairs
dc.identifier.urihttp://hdl.handle.net/10214/7488
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectglucose infusionen_US
dc.subjectdairy cowen_US
dc.subjectmammary protein synthesisen_US
dc.subjectS6K1en_US
dc.subject4EBP1en_US
dc.titleMammary mTOR activity during intravenous glucose infusions into lactating dairy cowsen_US
dc.typeThesisen_US

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