The Effects of Dexamethasone on Estrogen Cell Signaling Pathways in the mHippoE-14 Cell Line & Female Rats

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dc.contributor.advisorMacLusky, Neil
dc.contributor.authorDobronyi, Sydney
dc.date.accessioned2023-01-11T14:32:31Z
dc.date.available2023-01-11T14:32:31Z
dc.date.copyright2023-01
dc.date.created2023-01-06
dc.degree.departmentDepartment of Biomedical Sciencesen_US
dc.degree.grantorUniversity of Guelphen
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeBiomedical Sciencesen_US
dc.description.abstractPrevious research in our lab has demonstrated alterations in molecular markers of hippocampal structure and function in response to stress, including DUSP6 expression, as well as ERK and JNK phosphorylation (Nicholson 2019, Craig 2021, Pacosz 2021). To further characterize JNK phosphorylation, ERK phosphorylation, and DUSP6 expression we ran two experiments, one in the mHippoE-14 cell line, and one in cycling female rats in metestrus. A significant downregulation of p54 JNK phosphorylation was found in the mHippoE-14 cell line in response to the combination of 17β-estradiol (E2) pretreatment and dexamethasone treatment, although no significant effects were found in the cycling female rats. Altogether, the data from this thesis suggests that circulating levels of E2 in metestrus are not sufficient to reverse or attenuate glucocorticoid induced effects on DUSP6 expression, ERK phosphorylation, and JNK phosphorylation.en_US
dc.identifier.urihttps://hdl.handle.net/10214/27413
dc.language.isoenen_US
dc.publisherUniversity of Guelphen
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectMajor Depressive Disorderen_US
dc.subjectCell Signalingen_US
dc.subjectEstrogensen_US
dc.subjectStressen_US
dc.subjectHypothalamic-pituitary-adrenal Axisen_US
dc.subjectGlucocorticoidsen_US
dc.subjectDexamethasoneen_US
dc.subjectHippocampusen_US
dc.subjectBrain-Derived Neurotrophic Factoren_US
dc.subjectJNKen_US
dc.subjectERKen_US
dc.subjectDUSP6en_US
dc.titleThe Effects of Dexamethasone on Estrogen Cell Signaling Pathways in the mHippoE-14 Cell Line & Female Ratsen_US
dc.typeThesisen
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