Effect of the Parasitic Mite Varroa Destructor on the Immune System of Africanized and European Honey Bees at the Molecular and Cellular Levels

dc.contributor.advisorGuzman-Novoa, Ernesto
dc.contributor.advisorGoodwin, Paul H.
dc.contributor.authorKoleoglu, Gun
dc.date.accessioned2014-05-06T13:29:49Z
dc.date.available2014-11-01T05:00:12Z
dc.date.copyright2014-04
dc.date.created2014-03-12
dc.date.issued2014-05-06
dc.degree.departmentSchool of Environmental Sciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeEnvironmental Sciencesen_US
dc.description.abstractVarroa destructor parasitism of honeybees includes cuticle penetration and injection of saliva possibly containing immune-suppressing effectors. To replicate these effects on adults and pupae of European and Africanized honey bees, parasitism was compared to wounding, injecting buffer and injecting V. destructor homogenate. For both developmental stages and genotypes, wounding and injecting buffer increased expression of the TOLL and Imd immune pathway genes, defensin-1 and hymenoptaecin, but decreased expression of pUf68 (JAK/STAT immune pathway) and vitellogenin (longevity marker). However, the expressions of all genes studied decreased with homogenate injection similar to parasitism. For adults, haemocyte concentration and prophenol oxidase expression showed similar responses demonstrating similar effects on cellular and molecular immunity. Differences between European and Africanized honey bees, were much less than those observed between developmental stages. These results indicate that various aspects of V. destructor parasitism have different effects on immune responses, but the overall effect is immune suppression.en_US
dc.identifier.urihttp://hdl.handle.net/10214/8054
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectHoneybeeen_US
dc.subjectApis Melliferaen_US
dc.subjectgene expressionen_US
dc.subjectvarroa destructoren_US
dc.subjectimmuneen_US
dc.subjectimmunosuppressionen_US
dc.titleEffect of the Parasitic Mite Varroa Destructor on the Immune System of Africanized and European Honey Bees at the Molecular and Cellular Levelsen_US
dc.typeThesisen_US

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