Canada’s Food Guide is an important knowledge translation tool that provides Canadians guidance on what and how they should eat. Despite its’ importance, many Canadians do not use the Food Guide, and may not understand it. The objective of this thesis was to compare the perceptions and opinions of the 2007 and 2019 Canada’s Food Guide among 327 parents from the Guelph Family Health Study. To compare parents’ awareness and knowledge of the 2007 and 2019 Canada’s Food Guides Pearson’s Chi-Square was used. To compare parents’ opinions of the two Canada’s Food Guides Wilcoxon Rank-Sum tests were used. Thematic analysis was used to analyze parents’ open-ended comments about Canada’s Food Guides. Analysis revealed that parents had a high level of awareness of both Food Guides, significantly greater knowledge of 2019 Food Guide proportion recommendations, and more favourable opinions of the 2019 Food Guide compared to the 2007 Food Guide.