Characterization of the Non-Darkening Gene in a Cranberry Bean (Phaseolus vulgaris L.) Background

dc.contributor.advisorPauls, Peter
dc.contributor.authorRobson, Dana
dc.date.accessioned2017-09-05T18:56:19Z
dc.date.available2017-09-05T18:56:19Z
dc.date.copyright2017-07
dc.date.created2017-07-18
dc.date.issued2017-09-05
dc.degree.departmentDepartment of Plant Agricultureen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmePlant Agricultureen_US
dc.description.abstractSeveral common bean (Phaseolus vulgaris L.) market classes, including cranberry bean, demonstrate seed coat post-harvest darkening (PHD). PHD is associated with poor quality, lowering the overall value of the bean. A candidate MYB was sequenced in darkening (RD) and non-darkening (ND) cranberry bean lines to determine any differences between them. The RD and ND lines were identical for the candidate MYB. An F2 population of a cross between Etna (RD) and Witrood (ND) was created to study the inheritance of the PHD trait and identify its genomic location. The F2 lines were genotyped using Single Nucleotide Polymorphism (SNP) markers and used to develop a linkage map with 11 linkage groups. Multiple QTL Mapping (MQM) identified two QTL associated with colour parameter values on chromosome 10. SNP markers identified with the ND loci may be used in the future for marker assisted selection to develop ND cultivars.en_US
dc.description.sponsorshipHensall District Co-operative Inc.
dc.description.sponsorshipOntario Ministry for Agriculture Food and Rural Affairs
dc.description.sponsorshipOntario Bean Growers
dc.description.sponsorshipAgriculture and Agri-Food Canada
dc.description.sponsorshipOntario Ministry of Research and Innovation
dc.identifier.urihttp://hdl.handle.net/10214/11516
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectbeanen_US
dc.subjectpost harvest darkeningen_US
dc.subjectnon-darkeningen_US
dc.subjectSNPen_US
dc.subjectlinkageen_US
dc.subjectQTLen_US
dc.titleCharacterization of the Non-Darkening Gene in a Cranberry Bean (Phaseolus vulgaris L.) Backgrounden_US
dc.typeThesisen_US

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