Aptasensor Development For Biomarker Detection Using Fluorescent Guanine Quadruplexes

dc.contributor.advisorManderville, Richard A.
dc.contributor.authorBlanchard, Darian
dc.date.accessioned2015-07-30T18:38:17Z
dc.date.available2016-07-22T05:00:45Z
dc.date.copyright2015-07
dc.date.created2015-07-22
dc.date.issued2015-07-30
dc.degree.departmentDepartment of Chemistryen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeChemistryen_US
dc.description.abstractAptamers are nucleic acids that are found to bind molecular targets with a high degree of specificity and affinity. Guanine (G) rich aptamers are known to fold into G quadruplex structures (GQ). The synthesis of fluorescently modified guanine probes, 8-(4ʹʹ-acetylstyryl)-2ʹ-dG (AcVdG) and 8-((2-benzo[b]thiophen-2-yl)vinyl)-2ʹ-dG VBTdG, are presented in this thesis. The synthesized 8-vinyl-aryl-probes were incorporated into the TBA using solid phase oligonucleotide synthesis. Their impact on DNA structures were explored as was the ability of the probes to be used for the fluorescent detection of Pb2+, Sr2+, and Ba2+. Furthermore, to utilize the benefits of 8-aryl-dG and 8-vinyl-aryl-dG probes, both were incorporated into the TBA. The two probes act as Fluorescent Resonance Energy Transfer (FRET) pairs; one was used as the “switch” in the system, sensitively detecting GQ folding, whiles the other acts as “the signal” or “light” producing a detectable signal in the visible region.en_US
dc.description.sponsorshipGrain Farmers of Ontario
dc.description.sponsorshipGrowing Forward 2
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipOntario Government
dc.identifier.urihttp://hdl.handle.net/10214/8990
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectFluorescent Probesen_US
dc.subjectSynthetic DNAen_US
dc.subjectModified nucleobasesen_US
dc.subjectGuanine Quadruplexesen_US
dc.subjectToxin Detectionen_US
dc.subjectAptamersen_US
dc.titleAptasensor Development For Biomarker Detection Using Fluorescent Guanine Quadruplexesen_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Blanchard_Darian_201507_Msc.pdf
Size:
5.21 MB
Format:
Adobe Portable Document Format