Perinuclear localization of slow troponin C mRNA in muscle cells is controlled by a cis-acting element located in the 3' untranslated region

dc.contributor.advisorBag, Jnanankur
dc.contributor.authorOitomen, Ferry Martins
dc.date.accessioned2020-12-04T16:03:10Z
dc.date.available2020-12-04T16:03:10Z
dc.date.copyright2003
dc.degree.departmentDepartment of Molecular Biology and Geneticsen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractWe have investigated whether different mRNAs of the thin filament are localized within a specific cytoplasmic compartment of muscle cells. It was postulated that co-localization of multiple thin filament mRNAs may allow the synthesis of the thin filament proteins within the same vicinity, which will enhance rapid assembly of the thin filament. This process would be more energy efficient compared to if the proteins were randomly diffused. We have investigated the cytoplasmic localization of [alpha]-actin, slow skeletal troponin C (sTnC) and [beta]-actin mRNAs in cultured mouse C2C12 myotubes, using in situ hybridization techniques. To address the mechanism of perinuclear localization of sTnC mRNA, we have characterized the 'cis'-acting localization signal of the sTnC mRNA. We have mapped the localization signal within a 40 nucleotide-long region of the 3'UTR. To further investigate how the localization signal works in vivo, we have studied its interaction with trans-acting factor(s) and found that a 40 kDa protein of muscle cells bind specifically to the localization signal of sTnC mRNA. (Abstract shortened by UMI.)en_US
dc.identifier.urihttps://hdl.handle.net/10214/22465
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectmuscle cellsen_US
dc.subjectmRNAen_US
dc.subjectthin filamenten_US
dc.subjectcytoplasmic compartmenten_US
dc.subjectperinuclear localizationen_US
dc.titlePerinuclear localization of slow troponin C mRNA in muscle cells is controlled by a cis-acting element located in the 3' untranslated regionen_US
dc.typeThesisen_US

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