The Use of Midazolam, Isoflurane, and Nitrous Oxide for Sedation and Anesthesia of Ball Pythons (Python regius)

dc.contributor.advisorBienzle, Dorothee
dc.contributor.advisorNemeth, Nicole
dc.contributor.authorB. Larouche, Cédric
dc.date.accessioned2019-08-26T20:39:15Z
dc.date.available2019-08-26T20:39:15Z
dc.date.copyright2019-08
dc.date.created2019-08-06
dc.date.issued2019-08-26
dc.degree.departmentDepartment of Pathobiologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameDoctor of Veterinary Scienceen_US
dc.degree.programmeVeterinary Scienceen_US
dc.description.abstractThe objectives of this project were to characterize the pharmacodynamics and pharmacokinetics of midazolam in the ball python (Python regius), and to evaluate the effects of midazolam and nitrous oxide (N2O) on the minimum anesthetic concentration of isoflurane (MACIso) in this species. The pharmacodynamics of midazolam were evaluated in a blinded, randomized, crossover study comparing two dosages (1 and 2 mg/kg intramuscular [IM]) in ten ball pythons. There were no significant differences between the two dosages for any parameter evaluated. Midazolam provided moderate sedation and muscle relaxation in all snakes starting 10-15 min post-injection and lasting up to 3-5 days, with a peak effect 60 min post-injection. Heart rates were significantly lower than baseline from 30 min to 5.3 days post-injection with both dosages. In an open trial using 9 ball pythons, the reversal effect of flumazenil was evaluated by administering 0.08 mg/kg IM 60 min after the administration of 1 mg/kg IM of midazolam. Sedation and muscle relaxation were fully reversed within 10 min of administration, but all snakes fully resedated within 3 h. In a semi-blinded, randomized, crossover trial, nine ball pythons were used to evaluate the effects of 1 mg/kg IM midazolam and N2O mixed 50:50 with oxygen on the MACIso. Midazolam significantly decreased the MACIso from 1.11% (95% confidence interval [CI], 0.94-1.28%) to 0.48% (95% CI, 0.29-0.67%), and N2O decreased the MACIso to 0.92% (95% CI, 0.74-1.09%). No clinically relevant differences between treatments were noted regarding blood gases but isoflurane induction with face mask caused marked acidemia that resolved with mechanical ventilation. Central venous catheters were surgically placed in twelve ball pythons to evaluate the pharmacokinetics of midazolam. Snakes were randomly and equally divided into two groups receiving 1 mg/kg of midazolam either intracardiac (IC; group 1) or IM (group 2). Plasma concentrations of midazolam and its major metabolite 1-hydroxymidazolam were determined using high-performance liquid chromatography tandem-mass spectrometry. Mean bioavailability following IM administration was 89%. Mean ± SD clearance of midazolam was 0.053 ± 0.008 L/h/kg, leading to slow elimination half-lives (12.04 ± 3.25 h [IC] and 16.54 ± 7.10 h [IM]).en_US
dc.description.sponsorshipToronto Zoo
dc.description.sponsorshipPet Trust
dc.description.sponsorshipAndrea Leger Dunbar Summer Research Studentship
dc.identifier.urihttp://hdl.handle.net/10214/16951
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectmidazolamen_US
dc.subjectnitrous oxideen_US
dc.subjectisofluraneen_US
dc.subjectball pythonen_US
dc.subjectpython regiusen_US
dc.subjectsnakeen_US
dc.subjectreptileen_US
dc.subjectanesthesiaen_US
dc.subjectsedationen_US
dc.subjectminimum anesthetic concentrationen_US
dc.subjectMACen_US
dc.subjectminimum alveolar concentrationen_US
dc.subjectpharmacodynamicsen_US
dc.subjectpharmacokineticsen_US
dc.subjectroyal pythonen_US
dc.subjectinhalational anesthesiaen_US
dc.subject1-hydroxymidazolamen_US
dc.subjectsedativeen_US
dc.subjectanestheticen_US
dc.titleThe Use of Midazolam, Isoflurane, and Nitrous Oxide for Sedation and Anesthesia of Ball Pythons (Python regius)en_US
dc.typeThesisen_US

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