Exercise-induced net and unidirectional potassium transport in human erythrocytes

dc.contributor.advisorLindindger, M.I.
dc.contributor.authorGrudzien, Simon Paul
dc.date.accessioned2020-12-03T15:26:29Z
dc.date.available2020-12-03T15:26:29Z
dc.date.copyright2000
dc.degree.departmentDepartment of Human Biology and Nutritional Sciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractThe hypothesis was tested that exercise-induced changes in plasma composition stimulate unidirectional K+ (JinK) and net K+ (JnetK) transport in human red blood cells (RBCs). Experiment 1 (7 males, 2 females), used an exercise simulated (ES) plasma (resting plasma manipulated to mimic exercise plasma by increasing osmolality, [K+], [H +], [lactate] and [epinephrine] to exercise levels) to incubate resting RBCs and a K+ selective electrode to continuously measure JnetK. Experiment 2 (7 males, 4 females), utilized true exercise plasma (TEP) (obtained during a 30 s high intensity leg cycling exercise) to determine JinK via RBC 86Rb uptake and JnetK. The JnetK of Resting RBCs in experiment 1 was significantly elevated from 0.9 ± 28.7 meq · L RBCs -1 · hr-1 in resting plasma to 384.8 ± 164.3 meq · L RBCs-1 · hr -1 in ES plasma and in experiment 2 from -5.79 ± 16.09 meq · L RBCs-1 · hr-1 to 177.87 ± 59.87 meq · L RBCs-1 · hr-1 at 10s of incubation in TEP. JinK for resting RBCs in TEP was significantly elevated from control to 29 899 ± 8 680 [mu]mol · L RBCs-1 · hr-1 at 10 s. Peak JinK and JnetK occurred at the onset of incubation due to the Na +-K+ pump, Na+-K+-2Cl - cotransporter, and diffusion. It is concluded RBCs increased K+ transport in response to exercise-induced changes in plasma composition.en_US
dc.identifier.urihttps://hdl.handle.net/10214/21783
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectexercise-induced changesen_US
dc.subjectplasmaen_US
dc.subjectcompositionen_US
dc.subjectstimulateen_US
dc.subjectunidirectional transporten_US
dc.subjectnet transporten_US
dc.subjectpotassiumen_US
dc.subjecthumanen_US
dc.subjectred blood cellsen_US
dc.titleExercise-induced net and unidirectional potassium transport in human erythrocytesen_US
dc.typeThesisen_US

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