UV-C inactivation kinetics modelling of dairy-associated listeria monocytogenes strains and the effect of suspended solids on fluence response of listeria monocytogenes in milk using collimated beam UC-C device

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Handy, Stephanie
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University of Guelph

There is a disagreement on the UV C fluence required for microbial inactivation due to various understanding of UV-C fluence and its measurement methods. This study determined the UV-C fluences required for dairy-associated Listeria monocytogenes strains inactivation using a collimated beam device. In PBS, a UV-C fluence of 10 mJ/cm2 was sufficient to inactivate 5 logs of L. monocytogenes. Milk fat and protein concentration, fat globule and casein micelle particle sizes strongly correlated with observed UV C absorption and scattering of the milk. These optical properties significantly affected the inactivation of L. monocytogenes. Taking into consideration the UV C absorption and reduced scattering coefficients of milk in the UV C fluence calculation, inactivation similar to that observed in PBS at various UV C doses could be achieved in goat and cow milk at various fat levels. This study demonstrated that the inclusion of the milk optical properties is critical for accurate UV C fluence calculations.

inactivation kinetic, Listeria monocytogenes, non-thermal, UV-C, dairy, Milk, collimated beam