Investigating and characterizing phoenixin and tachykinin receptor-3 in the reproductive axis of the chicken, Gallus gallus

dc.contributor.advisorBédécarrats, Grégoy
dc.contributor.authorSparling, Brandi
dc.date.accessioned2017-01-06T18:11:15Z
dc.date.available2017-01-06T18:11:15Z
dc.date.copyright2016
dc.date.created2015-12-22
dc.date.issued2017-01-06
dc.degree.departmentDepartment of Animal Biosciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeAnimal and Poultry Scienceen_US
dc.description.abstractThis thesis identifies two new players, phoenixin and tackykinin receptor-3, in the reproductive axis of the chicken. Phoenixin is known to upregulate pituitary gonadotropin-releasing hormone receptor (GnRH-R) messenger RNA (mRNA) and tachykinin receptor-3 to generate GnRH pulsatility in mammals. Through database search, we confirm that these neuroproteins are encoded in the chicken genome. The full-length mRNA sequences were identified by 5’ and 3’ rapid-amplification of complementary DNA ends and annotated. Phoenixin mRNA was detected and quantified in various tissues via conventional and semi-quantitative PCR, respectively (in diencephalon, pituitary, ovary, gut and heart). In the diencephalon and pituitary of different strain, phoenixin was differentially expressed with age. Tackykinin receptor-3 mRNA is differentially expressed across tissues (diencephalon>pituitary>ovary) and in an age-dependent manner in the pituitary. The function of phoenixin was explored through treatment of sexually immature primary chicken pituitary cells and, unlike rodents no independent modulation of GnRH-R-III/I was detected.en_US
dc.identifier.urihttp://hdl.handle.net/10214/10184
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectChickenen_US
dc.subjectPhoenixinen_US
dc.subjectTachykinin receptor-3en_US
dc.subjectmRNAen_US
dc.subjectReproductionen_US
dc.subjectKNDyen_US
dc.titleInvestigating and characterizing phoenixin and tachykinin receptor-3 in the reproductive axis of the chicken, Gallus gallusen_US
dc.typeThesisen_US

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Investigating chicken phoenixin & tachykinin receptor-3