Characterization of peroxisomal multivesicular body morphology and the role of host-cell and viral components in their biogenesis in plant and yeast cells

dc.contributor.advisorMullen, Robert T.
dc.contributor.advisorGreenwood, John S.
dc.contributor.authorGibson, Kimberley Hendrika
dc.date.accessioned2020-12-03T14:55:55Z
dc.date.available2020-12-03T14:55:55Z
dc.date.copyright2009
dc.degree.departmentDepartment of Molecular and Cellular Biologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractPeroxisome biogenesis is complex, involving a diverse array of intracellular pathways and mechanisms that mediate their biogenesis and cellular functions. Relevant to our understanding of peroxisome biogenesis is the utilization of peroxisomal membranes for viral genome replication as observed in plant cells infected by several members of the 'Tombusviridae' family of positive-strand RNA viruses; Tomato Bushy Stunt Virus (TBSV), for instance, usurps an array of host factors that facilitate the transformation of peroxisomes into peroxisomal multivesicular bodies (PMVB) the sites of viral RNA replication. In this study, pMVB topology and biogenesis was investigated using transmission electron and epitluorescence microscopy of tobacco and wildtype or mutant budding yeast that were transformed with TBSV replicase proteins and a defective interfering viral RNA. Overall, the results suggest that host-virus interactions specifically associated with Endosomal Sorting Complex Required for Transport (ESCRT) and lipid metabolism are involved in TBSV replication and pMVB biogenesis.en_US
dc.identifier.urihttps://hdl.handle.net/10214/21647
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectperoxisome biogenesisen_US
dc.subjectperoxisomal membranesen_US
dc.subjectviral genome replicationen_US
dc.subjectTombusviridaeen_US
dc.subjectTomato Bushy Stunt Virusen_US
dc.subjectTBSVen_US
dc.subjectperoxisomal multivesicular bodiesen_US
dc.subjectPMVBen_US
dc.subjectpMVB topologyen_US
dc.subjectbiogenesisen_US
dc.subjectTBSV replicase proteinen_US
dc.subjectviral RNAen_US
dc.subjectplant cells|yeast cellsen_US
dc.titleCharacterization of peroxisomal multivesicular body morphology and the role of host-cell and viral components in their biogenesis in plant and yeast cellsen_US
dc.typeThesisen_US

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