Characterization of peroxisomal multivesicular body morphology and the role of host-cell and viral components in their biogenesis in plant and yeast cells

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Gibson, Kimberley Hendrika
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University of Guelph
Abstract

Peroxisome biogenesis is complex, involving a diverse array of intracellular pathways and mechanisms that mediate their biogenesis and cellular functions. Relevant to our understanding of peroxisome biogenesis is the utilization of peroxisomal membranes for viral genome replication as observed in plant cells infected by several members of the 'Tombusviridae' family of positive-strand RNA viruses; Tomato Bushy Stunt Virus (TBSV), for instance, usurps an array of host factors that facilitate the transformation of peroxisomes into peroxisomal multivesicular bodies (PMVB) the sites of viral RNA replication. In this study, pMVB topology and biogenesis was investigated using transmission electron and epitluorescence microscopy of tobacco and wildtype or mutant budding yeast that were transformed with TBSV replicase proteins and a defective interfering viral RNA. Overall, the results suggest that host-virus interactions specifically associated with Endosomal Sorting Complex Required for Transport (ESCRT) and lipid metabolism are involved in TBSV replication and pMVB biogenesis.

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Keywords
peroxisome biogenesis, peroxisomal membranes, viral genome replication, Tombusviridae, Tomato Bushy Stunt Virus, TBSV, peroxisomal multivesicular bodies, PMVB, pMVB topology, biogenesis, TBSV replicase protein, viral RNA, plant cells|yeast cells
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