The objective of project 9 was to determine and assess the relationship between concentrations of selected heavy metals in stream waters, suspended sediments, bottom sediments and soils within selected agricultural watersheds, draining into the lower Great Lakes, with the aim of elucidating sources, and storage and transport mechanisms of these elements. In support of these goals, the main objective of the investigation reported here, was to obtain reliable analytical information regarding concentrations of Cu, Zn, Cd and Pb in waters and suspended sediments from the six watersheds: AG1 (Big Creek), AG3 (Upper Little Ausable River), AG4 (Canagagigue), AG5 (Holiday Creek, tributary of Middle Thames River), AG10 (North Creek, tributary of Twenty Mile Creek), and AG13 (west branch of Hillman Creek).