Micropropagation and Cryopreservation of Endangered Plant Species- Draba yukonensis and Cirsium hillii. (Canby) Fernald
This study utilized in-vitro techniques for long-term conservation and revival of the threatened species, Hill’s Thistle (Cirsium hillii. (Canby) Fernald) and Yukon Draba (Draba yukonensis). Micropropagation protocols were developed from seed derived shoots. The cryopreservation protocol for in-vitro shoot tips of Hill’s thistle was established using the droplet-vitrification method which produced 90% shoot tips regrowth. The plants from cryopreserved shoot buds exhibited comparable or better survival and growth in the field compared to plants from non-cryopreserved shoot buds when transplanted to their natural habitats in the Bruce Peninsula National Park, Ontario. The Yukon Draba plants were successfully micropropagated and acclimatized in the greenhouse. Droplet-vitrification method used to optimize cryopreservation protocol for Yukon Draba using in-vitro shoot tips which resulted in 100 % survival and 66 % regrowth. Collectively, these results provide evidence of the successful application of in-vitro technologies for ex-situ conservation and recovery of endangered plant species.