An experiment that involved 79 named cultivars and advanced selections of Fragaria × ananassa L., 46 Fragaria virginiana Duch. clones, and 12 F. virginiana backcross selections, and eight Fragaria virginiana × Fragaria chiloensis(L.) Duch. (all Rosaceae) selections, was conducted to detect variation in strawberry genotypes for resistance to tarnished plant bug, Lygus lineolaris(Palisot de Beauvois) (Heteroptera: Miridae). The F. virginiana genotypes were shown to be more resistant than the cultivars and advanced selections in both 2001 and 2002. Within the group of cultivars and advanced selections, several June bearing and dayneutral genotypes were more resistant than others. There were fewer plant bugs on F. virginiana than on the cultivars and hybrids. Insect numbers were consistently correlated with percentage of damaged fruits and damage severity, but total numbers of flowers and fruits were only correlated with insect numbers, percentage fruit damage, and damage severity in 1 of the 2 years. Our results suggest that strawberry cultivars, highly resistant to tarnished plant bug, can be bred, if the trait is introgressed from F. virginiana selections.