This thesis investigates the effect of environment on energy metabolism when cats underwent acclimation to indirect calorimetry chambers, as well as the effects of a novel to cats micronutrient supplement – policosanol – on lipid profile and energy metabolism. Acclimation to indirect calorimetry chambers found a decrease in energy expenditure and macronutrient metabolism at the end of acclimation compared to the beginning. Policosanol supplementation was found to be safe for feline consumption, however, no significant changes were found on lipid profile and energy metabolism. These results suggest that previous indirect calorimetry studies not utilizing acclimation are overestimating energy metabolism, and that future research should use a similar acclimation procedure to ensure accurate data collection. Furthermore, as policosanol supplementation in domestic cats was found to be safe similar to previous research, future research should be performed utilizing a different experimental design and population of cats to determine proof of principle and positive effects.