T lymphocyte responses to Mycobacterium avium subspecies paratuberculosis (Map)-derived culture filtrate proteins and membrane vesicles during early intestinal Map infection in calves

dc.contributor.advisorPlattner, Brandon
dc.contributor.authorEl Skhawy, Nahla
dc.date.accessioned2016-09-07T18:28:54Z
dc.date.available2017-08-30T05:00:19Z
dc.date.copyright2016-08
dc.date.created2016-08-25
dc.date.issued2016-09-07
dc.degree.departmentDepartment of Pathobiologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmePathobiologyen_US
dc.description.abstractJohne’s disease (JD) is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subspecies paratuberculosis (Map). T lymphocytes play a significant role in JD control; however anti‐mycobacterial T cell responses during early intestinal Map infection remain poorly understood. Crude Map antigen preparations have been studied to detect T cell responses during subclinical JD; however, use of secreted Map antigens remains unexplored. Our hypothesis was that exposure of Map sensitized T cells to Map-secreted antigens would evoke T cell responses that could be assessed by flow cytometry and ELISA. We screened T lymphocytes from blood of experimentally Map-infected calves against Map whole cell sonicate, Map culture filtrate and Map membrane vesicles. Our data supported the hypothesis and secreted IFNγ was detected in supernatants of cells from only Map-infected calves upon exposure to Map antigens. Results demonstrated that CD4 T cells from Map-infected calves were a source of IFNγ produced in response to Map antigens. CD4 and WC1+ γδ T cells from Map infected calves and WC1- γδ T cells from uninfected calves proliferated after stimulation with Map antigens, which was consistent with the previous findings.en_US
dc.description.sponsorshipOntario Ministry of Agriculture, Food and Rural Affairs
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipOntario Graduate Fellowship
dc.identifier.urihttp://hdl.handle.net/10214/9956
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rightsAttribution-NonCommercial 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/2.5/ca/*
dc.subjectParatuberculosisen_US
dc.subjectMAPen_US
dc.subjectMembrane vesiclesen_US
dc.subjectCulture filtrateen_US
dc.subjectT lymphocyte responsesen_US
dc.titleT lymphocyte responses to Mycobacterium avium subspecies paratuberculosis (Map)-derived culture filtrate proteins and membrane vesicles during early intestinal Map infection in calvesen_US
dc.typeThesisen_US

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