Bioremediation of Hydrocarbon Contaminants in Subsurface Groundwater
Hydrocarbons are common contaminants in the subsurface as a result of spills caused by the petroleum and chemical industry. Current remediation techniques require exhaustive construction to either excavate contaminated soil to be treated ex-situ or to install impermeable barriers to contain contaminants preventing plume migration or permeable barriers to treat in-situ. This thesis will explore two novel approaches that simultaneously contain and bioremediate naphthalene, a model hydrocarbon, without the requirement of traditional barrier installation. The first part of this study utilizes a pumpable double emulsion system that envelops Pseudomonas sp. cells that gels in the subsurface creating an injectable reactive barrier in the subsurface. The remainder of this thesis looks at the sorption properties of naphthalene on zein substrates as a method of sequestering PAHs.