The Effects of Oil Sands Process-Affected Waters and their Associated Constituents on Fathead Minnow (Pimephales promelas) Reproductive Physiology

dc.contributor.advisorVan Der Kraak, Glen
dc.contributor.authorKavanagh, Richard James of Integrative Biologyen_US of Guelphen_US of Philosophyen_US Biologyen_US
dc.description.abstractAs part of their reclamation plan, oil sands operators propose to transfer the mature fine tailings, which are a by-product of the oil sands extraction process, to open-pits and cap them with either a layer of surface water or oil sands process-affected waters (OSPW). These oil sands pit lakes are expected to develop habitats with productive capabilities comparable to natural lakes in the region. The studies presented in this thesis evaluate the potential impact of OSPW and its associated constituents [i.e. acid-extractable organics (e.g. naphthenic acids; NAs) and salts] on the reproductive physiology of adult fathead minnow (Pimephales promelas). Through 14-21 day fathead minnow reproduction assays it was demonstrated that aged OSPW can impair spawning, lower plasma sex steroid concentrations, and reduce male secondary sexual characteristics. The acid-extractable organics in OSPW were demonstrated to have an adverse effect on fathead minnow reproductive physiology. Other studies showed that the high salinity which characterizes OSPW also influences toxicity. When fathead minnows were exposed to the OSPW extract and 700 mg/l of NaHCO3, the NaHCO3 reduced the inhibitory effects of the extract on the numbers of reproductive tubercles and plasma testosterone levels by reducing the uptake of NAE to the fish. Embyro and larval bioassays also revealed that NaHCO3 reduces the acute toxic effects of the OSPW extract. An assessment of a wild population of fathead minnows inhabiting an OSPW pond determined that there were differences in the condition factor (CF), gonadosomatic indices (GSIs), liver somatic indices (LSIs), male secondary sexual characteristics, and 11-ketotestosterone concentrations in the fathead minnows from the OSPW pond relative to fish collected at reference sites. The opercula of fathead minnows from the OSPW pond also differed from those of reference fish and an examination of the gills revealed that were a number of proliferative and degenerative alterations relative to reference fish. Collectively, these studies demonstrate that aged OSPW has the potential to negatively affect the reproductive physiology of fathead minnows and suggest that aquatic habitats with high NAs concentrations (>10 mg/l) will have adverse effects on fish.en_US
dc.description.sponsorshipCanadian Natural Resources Ltd.
dc.description.sponsorshipCanadian Water Network
dc.description.sponsorshipCanadian Oil Sands Network for Research and Development
dc.description.sponsorshipImperial Oil Ltd.
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipShell Canada Energy
dc.description.sponsorshipSuncor Energy Inc.
dc.description.sponsorshipSyncrude Canada Ltd.
dc.description.sponsorshipTotal E&P Canada
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectoil sandsen_US
dc.subjectnaphthenic acidsen_US
dc.subjectfathead minnowen_US
dc.subjectendocrine disruptionen_US
dc.subjectprocess-affected wateren_US
dc.titleThe Effects of Oil Sands Process-Affected Waters and their Associated Constituents on Fathead Minnow (Pimephales promelas) Reproductive Physiologyen_US


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