Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome and characterization of Cf103, a zinc-finger containing ORF

dc.contributor.advisorKrell, Peter J.
dc.contributor.authorDe Jong, Jondavid
dc.date.accessioned2020-08-24T15:54:48Z
dc.date.available2020-08-24T15:54:48Z
dc.date.copyright2004
dc.degree.departmentDepartment of Microbiologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractThe dsDNA genome of 'Choristoneura fumiferana' nucleopolyhedrovirus (CfMNPV) was sequenced and analyzed in the context of other group I NPVs. The genome was 129 609 bp in length with a G + C content of 50.1%. A total of 146 ORFs of greater than 150 bp and with minimal overlap were identified. In addition, four homologous regions (hrs) were identified which contained 7-10 repeats with a 36 bp imperfect palindromic core. Overall, CfMNPV was most similar to OpMNPV based on gene content and arrangement and overall amino acid identity. Comparison with other completely sequenced baculovirus genomes identified eleven ORFs that were unique to CfMNPV. One of these, ' Cf103', encoded a protein with a putative zinc-finger domain. ' Cf103' was further characterized in terms of transcription and was found not to be essential for CfMNPV replication in tissue culture.en_US
dc.identifier.urihttps://hdl.handle.net/10214/20573
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectdsDNA genome |Choristoneura fumiferana nucleopolyhedrovirusen_US
dc.subjectsequenceen_US
dc.subjectgroup I NPVsen_US
dc.titleAnalysis of the Choristoneura fumiferana nucleopolyhedrovirus genome and characterization of Cf103, a zinc-finger containing ORFen_US
dc.typeThesisen_US

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