mRNA Destabilizing Factor TTP Expression at the Porcine Maternal-Fetal Interface

dc.contributor.advisorTayade, Chandrakant
dc.contributor.advisorMacLusky, Neil J.
dc.contributor.authorKhalaj, Kasra
dc.date.accessioned2013-09-07T19:52:39Z
dc.date.available2013-09-07T19:52:39Z
dc.date.copyright2013-09
dc.date.created2013-08-28
dc.date.issued2013-09-07
dc.degree.departmentDepartment of Biomedical Sciencesen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeBiomedical Sciencesen_US
dc.description.abstractThe tristetraprolin family consists of mRNA destabilizing genes that bind to target mRNA and degrade them. In this context, TIS11 gene family is of primary interest in the spontaneous fetal loss seen in pigs. I hypothesized that the tristetraprolin family regulates these genes in this model. Higher levels of the TIS11 family transcripts in endometrium and trophoblast samples from healthy conceptuses at gd20 were found than in tissues from arresting conceptus attachment sites and significantly higher levels of TIS11 family transcripts were expressed in trophoblast samples associated with arresting conceptuses at gd50 compared to healthy endometrium and trophoblast. TIS11 proteins were expressed and localized at both maternal and fetal sides of maternal-fetal interface. These results provide a basis for understanding some aspects of gene regulation by mRNA destabilizing factors such as tristetraprolin at the maternal-fetal interface and how these factors may play a role in porcine pregnancy and fetal loss.en_US
dc.description.sponsorshipDepartment of Biomedical Sciences
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipOntario Pork
dc.description.sponsorshipBioniche Life Sciences Inc.
dc.identifier.urihttp://hdl.handle.net/10214/7482
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectTTPen_US
dc.subjectPigen_US
dc.subjectTIS11ben_US
dc.subjectTIS11den_US
dc.subjectTNFaen_US
dc.subjectGMCSFen_US
dc.titlemRNA Destabilizing Factor TTP Expression at the Porcine Maternal-Fetal Interfaceen_US
dc.typeThesisen_US

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