This thesis investigated the effects of feeding frequency on plasma appetite-regulating hormones (ghrelin, GIP, GLP-1, leptin, and PYY), insulin, amino acids, and whole blood glucose in adult cats. In addition, activity, energy expenditure and respiratory quotient were assessed. Cats eating once daily had increased postprandial concentrations of amino acids, ghrelin, GIP, GLP-1, insulin, and PYY, whereas leptin and glucose did not differ. Activity was greater in cats consuming four meals. Fasting respiratory quotient was lower in cats eating one meal. Cats fed once daily consumed less food than cats fed more frequently. These results suggest feeding cats fewer meals over a longer period of time could cause weight loss as cats may be more satiated and are prone to consume less calories when fed fewer meals. Cats fed intermittently could have greater protein synthesis due to greater postprandial concentrations of amino acids and insulin.