Expression of RD29A cold responsive promoter elements in alfalfa, Medicago sativa L.

dc.contributor.advisorMcKersie, Bryan D.
dc.contributor.authorWang, Yang
dc.date.accessioned2021-04-23T12:17:55Z
dc.date.available2021-04-23T12:17:55Z
dc.date.copyright1997
dc.degree.departmentDepartment of Crop Scienceen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractA rd29a cold inducible promoter was introduced into alfalfa (Medicago sativa L.) by Agrobacterium transformation. The GUS activity driven by the rd29a promoter increased in leaf and stem, but decreased in the root when the transgenic alfalfa was placed at 2$\sp\circ$C. The GUS mRNA in stem tissue transiently increased within 2-3 days at 2$\sp\circ$C. One or three copy number of a truncated rd29a promoter containing the dehydration responsive element was transcriptionally fused in two orientations with GUS and firefly luciferase genes respectively. These constructs were introduced into alfalfa leaflets by particle bombardment. In luciferase activity assay, the Dual Luciferase Assay System (firefly and Renilla luciferase) was used. The activities of firefly, Renilla luciferases, and GUS were unstable under cold treatment. The alfalfa response to the rd29a promoter was difference observed in Arabidopsis and tobacco. The Dual Luciferase Assay System was unsuitable for investigation at cold in alfalfa.en_US
dc.identifier.urihttps://hdl.handle.net/10214/25280
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectRD29Aen_US
dc.subjectcold inducible promoteren_US
dc.subjectalfalfaen_US
dc.subjectMedicago sativa L.en_US
dc.subjectAgrobacteriumen_US
dc.subjecttransformationen_US
dc.titleExpression of RD29A cold responsive promoter elements in alfalfa, Medicago sativa L.en_US
dc.typeThesisen_US

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