Bacterial-binding properties and variability of ladderlectin in rainbow trout plasma

dc.contributor.advisorLumsden, John S.
dc.contributor.authorYoung, Karrie Marie
dc.date.accessioned2021-03-31T15:37:22Z
dc.date.available2021-03-31T15:37:22Z
dc.date.copyright2007
dc.degree.departmentDepartment of Pathobiologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.description.abstractLadderlectin is a putative defense protein originally isolated from rainbow trout serum. This thesis describes the bacterial-binding properties of rainbow trout ladderlectin (RT-LL), examines individual variability of RT-LL concentrations in healthy trout and temporal alterations of RT-LL concentration during the acute phase response (APR). Binding assays with intact bacteria demonstrated that RT-LL binds a range of relevant fish pathogens. Development of an indirect ELISA to measure plasma RT-LL concentrations revealed significant (' P' < 0.0001) variability between individual trout in two separate populations. During the APR, RT-LL concentrations did not increase in fish either injected with bacterial products or experimentally infected with ' Aeromonas salmonicida.' In contrast, a modest, but statistically significant ('P' < 0.05), reduction of RT-LL concentration was observed in experimentally infected fish. These studies demonstrate that RT-LL is variably expressed in plasma of rainbow trout and may decrease during clinical infection, but is not induced during the APR.en_US
dc.identifier.urihttps://hdl.handle.net/10214/24235
dc.language.isoen
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectladderlectinen_US
dc.subjectputative defense proteinen_US
dc.subjectrainbow trout serumen_US
dc.subjectbacterial-binding propertiesen_US
dc.subjectrainbow trout ladderlectinen_US
dc.subjectvariabilityen_US
dc.subjecttemporal alterationsen_US
dc.subjectacute phase responseen_US
dc.titleBacterial-binding properties and variability of ladderlectin in rainbow trout plasmaen_US
dc.typeThesisen_US

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